PUBLICATIONS
(see below for Reviews / Book Chapters)
2024
Jessen V. Bredeson, Austin B. Mudd, Sofia Medina-Ruiz, Therese Mitros, Owen Kabnick Smith, Kelly E. Miller, Jessica B. Lyons, Sanjit S. Batra, Joseph Park, Kodiak C. Berkoff, Christopher Plott, Jane Grimwood, Jeremy Schmutz, Guadalupe Aguirre-Figueroa, Mustafa K. Khokha, Maura Lane, Isabelle Philipp, Mara Laslo, James Hanken, Gwenneg Kerdivel, Nicolas Buisine, Laurent M. Sachs, Daniel R. Buchholz, Taejoon Kwon, Heidi Smith-Parker, Marcos Gridi-Papp, Michael J. Ryan, Robert D. Denton, John H. Malone, John B. Wallingford, Aaron F. Straight, Rebecca Heald, Dirk Hockemeyer, Richard M. Harland & Daniel S. Rokhsar. Conserved chromatin and repetitive patterns reveal slow genome evolution in frogs. Nat. Comm. 15:579. doi.org/10.1038/s41467-023-43012-9
2023
Paul, B., Dockery, R., Valverde, V.M., Buchholz, D.R. Characterization of a novel corticosterone response gene in Xenopus tropicalis tadpole tails. Front. Endocrinol. 14:1121002. doi: 10.3389/fendo.2023.1121002
2023
Sterner, Z.R., Jabrah, A., Shaidani, N.-I., Horb, M.E., Dockery, R., Paul, B., Buchholz, D.R. Development and metamorphosis in frogs deficient in the thyroid hormone transporter MCT8. Gen Comp. Endocrinol. 331:114179. doi.org/10.1016/j.ygcen.2022.114179
2022
Paul, B., Shewade, L.H., Buchholz, D.R. cyp21a2 knockout tadpoles survive metamorphosis despite low corticosterone. Endocrinol. 164:1-12. doi.org/10.1210/endocr/bqac182
2022
Rigolet, M., Buisine, N., Scharwatt, M., Duvernois-Berthet, E., Buchholz, D.R., Sachs, L.M. Crosstalk between thyroid hormone and corticosteroid signaling targets cell proliferation in Xenopus tropicalis tadpole liver. Int. J. Mol. Sci. 23:13715. doi.org/10.3390/ijms232213715
Zahn, N., James-Zorn, C.H., Ponferrada, V.G., Adams, D.S., Grzymkowski, J.K., Buchholz, D.R., Nascone-Yoder, N.M., Horb, M., Moody, S.A., Vize, P.D., and Zorn, A.M. Normal Table of Xenopus development: a new graphical resource. Development 149, dev200356. doi:10.1242/dev.200356
2022
Sterner, Z.R., Buchholz, D.R. Glucocorticoid receptor mediates corticosterone-thyroid hormone synergy essential for metamorphosis in Xenopus tropicalis tadpoles. Gen. Comp. Endocrinol. 315:113942. doi.org/10.1016/j.ygcen.2021.113942
2022
2020
Zlatow, A.L., Wilson, S., Bouley, D.M., Tetens-Woodring, J., Buchholz, D.R., Green, S.L. Axial Skeletal Malformations in Genetically Modified Xenopus laevis and Xenopus tropicalis. Comp. Med. 70:1-10.
2020
Shewade, L.H., Schoephoerster, J.A., Patmann, M.D., Kulkarni, S.S., Buchholz, D.R. Corticosterone is essential for survival through frog metamorphosis. Endocrinol. 161:1-15. doi.org/10.1210/endocr/bqaa193
2020
Hasebe, T., Fujimoto, K., Buchholz, D.R., Ishizuya-Oka, A. Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine. Gen. Comp. Endocrinol. 292: 113441. doi.org/10.1016/j.ygcen.2020.113441
2020
Sterner, Z.R., Shewade, L.H., Mertz, K.M., Sturgeon, S.M., Buchholz, D.R. Glucocorticoid receptor is required to survive through metamorphosis in the frog Xenopus tropicalis. Gen. Comp. Endocrinol. 291: 113419. https://doi.org/10.1016/j.ygcen.2020.113419
2019
Sterner, Z.R., Rankin, S.A, Wlizla, M., Choi, J.A., Luedeke, D.M., Zorn, A.M., Buchholz, D.R. Novel vectors for functional interrogation of Xenopus ORFeome coding sequences. genesis 2019:e23329. doi.org/10.1002/dvg.23329
2018
Schneider, K.A., Shewade, L.H., Buisine, N., Sachs, L.M., Buchholz, D.R. A novel stress hormone response gene in tadpoles of Xenopus tropicalis. Gen. Comp. Endocrinol. 260:107–114
2017
Kulkarni, S.S., Gomez-Mestre, I. Denver, R.J. Buchholz, D.R. Genetic accommodation of the larval period via modified endocrine signalling explains phenotypic divergence among spadefoot toad species. Nature Comm. 8:993. DOI: 10.1038/s41467-017-00996-5. Share link: http://rdcu.be/wTg2
2017
Patmann, M.D., Shewade, L.H., Schneider, K.A., Buchholz, D.R. Xenopus Tadpole Tissue Harvest. Cold Spring Harb Protoc; doi:10.1101/pdb.prot097675
2017
Shewade, L. Schneider, K., Brown, A., Buchholz, D.R. In-vivo regulation of Krüppel-like factor 9 by corticosteroids and their receptors across tissues in tadpoles of Xenopus tropicalis. Gen. Comp. Endocrinol. 248:79–86
2017
Choi, J., Ishizuya-Oka, A., Buchholz, D.R. Growth, development, and intestinal remodeling occurs in the absence of thyroid hormone receptor alpha in tadpoles of Xenopus tropicalis. Endocrinol. 158:1623-1633
2016
Budzik, K.A., Żuwała, K., Buchholz, D. R. Taste organ growth and development in the direct developing frog Eleutherodactylus coqui (Lissamphibia: Eleutherodactylidae). Acta Zool. 97:433-441
2015
Choi, J., Moskalik, C., Ng, A., Matter, S.F., Buchholz, D.R. Regulation of thyroid hormone-induced development in vivo by thyroid hormone transporters and cytosolic binding proteins. Gen. Comp. Endocrinol. 222:69–80
2015
Burraco, P., Arribas, R., Kulkarni, S.S., Buchholz, D.R., Gomez-Mestre, I. Comparing techniques for measuring corticosterone in tadpoles. Current Zoology 61: 835–845
2015
Choi, J., Suzuki, K.T., Sakuma, T., Shewade, L., Yamamoto, T., and Buchholz, D.R. Unliganded thyroid hormone receptor a regulates developmental timing via gene repression in Xenopus tropicalis. Endocrinology 156:735–744
2014
Haas, A., Pohlmeyer, J., McLeod, D.S., Kleinteich, T., Hertwig, S.T., Das, I., Buchholz, D.R. Extreme tadpoles II: The highly derived larval anatomy of Occidozyga baluensis (Boulenger, 1896), an obligate carnivorous tadpole. Zoomorphology 133:321–342
2013
Gomez-Mestre, I. Kulkarni, S., Buchholz, D.R. Mechanisms and Consequences of Developmental Acceleration in Tadpoles Responding to Pond Drying. PLoS One 8: e84266
2012
Kerney, R.R., Brittain, A.L. Hall, B.K., Buchholz, D.R. Cartilage on the move: Cartilage lineage tracing during tadpole metamorphosis. Dev. Growth, Differentiation 54:739-752
2012
Kulkarni, S.S. and Buchholz, D.R. Beyond Synergy: Corticosterone and thyroid hormone have numerous interaction effects on gene regulation in Xenopus tropicalis tadpoles. Endocrinol. 153(11):5309–5324
2011
Kulkarni, S.S., Gomes-Mestre, I., Moskalik, C.L., Storz, B.L., Buchholz, D.R. Evolutionary reduction of developmental plasticity in desert spadefoot toads. J. Evol. Biol. 24:2445–2455
2011
Hollar, A.R., Choi, J., Grimm, A.T., Buchholz, D.R. Higher thyroid hormone receptor expression correlates with short larval periods in spadefoot toads and increases metamorphic rate. Gen. Comp. Endocrinol. 173:190-198
2011
Rankin, S.A., Zorn, A.M., Buchholz, D.R. New doxycycline-inducible transgenic Xenopus. Dev. Dynamics 240:1467–1474
2011
Hasebe, T., Buchholz, D.R., Shi, Y.-B., Ishizuya-Oka, A. Epithelial-connective tissue interactions induced by thyroid hormone receptor are essential for adult stem cell development in the Xenopus laevis intestine. Stem Cells 29:154-161
2010
Kulkarni, S.A., Singamasetty, S., Buchholz, D.R. Corticotropin-releasing factor regulates the development in the direct developing frog, Eleutherodactylus coqui. Gen. Comp. Endocrinol. 169:225-230
2010
Heimeier, R.A., Das, B., Buchholz, D.R., Fioretino, M., Shi, Y.-B. Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult. Genome Biology 11:R55
2010
Buchholz, D.R., Hollar, A. R., Ledon-Retig, C. Scaphiopus couchii: developmental morphology. Herpetol. Rev. 41(4):480
2010
Hayes, T.B., Khoury, V., Narayan, A., Nazir, M., Park, A., Brown, T., Adame, L., Chan, E., Buchholz, D.R., Stueve, T., Gallipeau, S. Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis). PNAS 107:4612-4614
2009
Das, B., Heimeier, R.A., Buchholz, D.R., Shi, Y.-B. Identification of direct thyroid hormone response genes reveals the earliest gene regulation programs during frog metamorphosis. J. Biol. Chem. 284: 34167–34178
2009
Rankin, S.A., Hasebe, T., Zorn, A.M., Buchholz, D.R. Improved Cre reporter transgenic Xenopus. Dev. Dynamics238:2401-2408
2009
Ishizuya-Oka, A., Hasebe, T., Buchholz, D.R., Kajita, M. Fu, L. Shi, Y.-B. The origin of the adult intestinal stem cells induced by thyroid hormone in Xenopus laevis. FASEB J. 23:2568-2575
2009
Heimeier, R.A. Das, B., Buchholz, D.R., Shi, Y.-B. The xenoestrogen bisphenol A inhibits postembryonic vertebrate development by antagonizing gene regulation by thyroid hormone. Endocrinology 150(6):2964-2973
2007
Sato, Y. Buchholz, D.R., Paul, B.D., Shi, Y.-B. A role of unliganded thyroid hormone receptor in postembryonic development in Xenopus laevis. Mech. Develop. 124:476-488
2007
Buchholz, D.R., Singamsetty, S., Karadge, U., Williamson, S., Langer, C.E., Elinson, R.P. Nutritional endoderm in a direct developing frog: A potential parallel to the evolution of the amniote egg. Dev. Dynamics 236:1259-1272
2007
Paul, B.D., Buchholz, D.R., Fu, L., Shi, Y.-B. SRC-p300 coactivator complex is required for thyroid hormone-induced amphibian metamorphosis. J. Biol. Chem. 282(10):7472-7481
2007
Buchholz, D.R., Heimeier, R.A., Das, B., Washington, T., Shi, Y.-B. Pairing morphology with gene expression in thyroid hormone-induced intestinal remodeling and identification of a core set of TH-induced genes across tadpole tissues. Devel. Biol. 303:567-590
2006
Goodward, D.M., Barber, P.H., Buchholz, D.R. Geographic Distribution. Scaphiopus couchii. Herpetol. Rev. 37(3): 359-360
2006
Gomez-Mestre, I. Buchholz, D.R. Developmental plasticity mirrors differences among taxa in spadefoot toads linking plasticity and diversity. Proc. Nat. Acad. Sci. 103(50): 19021-19026
2006
Fu, L., Tomita, A., Wang, Hua, Buchholz, D.R. Shi, Y.-B. Transcriptional regulation of the Xenopus laevis stromelysin-3 gene by thyroid hormone is mediated by a DNA element in the first introl. J. Biol. Chem. 281 (25): 16870-16878
2005
Buchholz, D.R., Paul, B.D., and Shi, Y.-B. Gene-specific changes in promoter occupancy by thyroid hormone receptor during frog metamorphosis: Implications for developmental gene regulation. J. Biol. Chem. 280 (50): 41222-41228
2005
Fu, L., Ishizuya-Oka, A., Buchholz, D.R., Amano, T., Matsuda, H., and Shi, Y.-B. A causative role of stromelysin-3 in extracellular matrix remodeling and epithelial apoptosis during intestinal metamorphosis in Xenopus laevis. J. Biol. Chem. 280 (30): 27856-27865
2005
Buchholz, D.R. and Hayes, T.B. Variation in thyroid hormone action and tissue content underlies species differences in the timing of metamorphosis in desert frogs. Evolution and Development 7(5): 458-467
2005
Paul, B.P., Buchholz, D.R., and Shi, Y.-B. Tissue- and gene-specific recruitment of steroid receptor coactivator-3 by thyroid hormone receptor during development. J. Biol. Chem. 280 (29): 27165-27172
2005
Paul, B.P., Fu, L., Buchholz, D.R., and Shi, Y.-B. Coactivator recruitment is essential for liganded thyroid hormone receptor to initiate amphibian metamorphosis. Mol. Cell. Biol. 25 (13): 5712-5724
2004
Buchholz, D.R., Tomita, A., Fu, L., Paul, B.D., and Shi, Y.-B. Transgenic analysis reveals that thyroid hormone receptor is sufficient to mediate the thyroid hormone signal in frog metamorphosis. Mol. Cell. Biol. 24 (20): 9026-9037
2004
Buchholz, D.R., Ishizuya-Oka, A., and Shi, Y.-B. Spatial and temporal expression pattern of a novel gene in the frog Xenopus laevis: correlations with adult intestinal epithelial differentiation during metamorphosis. Mech. Devel./Gene Expression Patterns 4: 321-328
2004
Tomita, A., Buchholz, D.R., and Shi, Y.-B. Recruitment of N-CoR/SMRT-TBLR1 corepressor complex by unliganded thyroid hormone receptor for gene repression during frog development. Mol. Cell. Biol. 24 (8): 3337-3346
2004
Buchholz, D.R., Fu, L., and Shi, Y.-B. Cryopreservation of Xenopus transgenic lines. Molec. Reprod. Devel. 67: 65-69
2003
Buchholz, D.R., Hsia, V., Fu, L., and Shi, Y.-B. A dominant negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes. Mol. Cell. Biol. 23 (19): 6750-6758
2003
Tomita, A., Buchholz, D.R., Obata, K., and Shi, Y.-B. Fusion protein of retinoic acid receptor a with promyelocytic leukemia protein of promyelocytic leukemia zinc finger protein recruits N-CoR-TBLR1 corepressor complex to repress transcription in vivo. J. Biol. Chem. 278 (33): 30788-30795
2003
Garcia-Paris, M., Buchholz, D.R.,  and Parra-Olea, G. Phylogenetic relationships of Pelobatoidea re-examined using mtDNA. Mol. Phylogenet. Evol. 28 (1): 12-23
2002
Fu*, L., Buchholz*, D.R., and Shi, Y.-B. Novel double-promoter approach for identification of transgenic animals: a tool for in vivo analysis of gene function and development of gene-based therapies. Molec. Reprod. Devel. 62 (4): 470-476. *equal contributors
2002
Buchholz, D.R. and Hayes, T.B. Evolutionary patterns of diversity in spadefoot toad metamorphosis (Anura:Pelobatidae).Copeia 2002(1): 180-189
2000
Buchholz, D.R. and Hayes, T.B. Larval period comparison for the spadefoot toads Scaphiopus couchii and Spea multiplicata (Anura: Pelobatidae). Herpetologica. 56 (4): 455-468
2000
Buchholz, D. R. and Ely, E. Geographic Distribution. Taricha torosa. Herpetol. Rev. 31(2): 108
1995
Buchholz, D.R., Scott, P., and Shastri, N. Presentation without proteolytic cleavage of endogenous precursors in the MHC class I antigen processing pathway. J. Biol. Chem. 270: 6515-22
1995
Malarkannan, S., Goth, S., Buchholz, D.R., and Shastri, N. The role of MHC class I molecules in the generation of endogenous peptide/MHC complexes. J. Immunol. 154: 585-98
1994
Ruben, L.N., Ahmadi, P., Johnson, R.O., Buchholz, D.R., Clothier, R.H. and Shiigi, S. Apoptosis in the thymus of developing Xenopus laevis. Devel. Comp. Immunol. 18(4): 343-52
1994
Ruben, L.N., Buchholz, D.R., Ahmadi, P., Johnson, R.O., Clothier, R.H., and Shiigi, S. Apoptosis in thymus of adult Xenopus laevis. Devel. Comp. Immunol. 18(4): 231-38
REVIEWS / BOOK CHAPTERS
2023
Paul, B., Buchholz, D.R. Minireview: Glucocorticoid-Leptin Crosstalk: Role of Glucocorticoid-Leptin Counter-regulation in Metabolic Homeostasis And Normal Development. Int. Comp. Biol. doi.org/10.1093/icb/icad119
2022
Paul, B., Sterner, Z.R., Buchholz, D.R., Shi, Y.-B., Sachs, L.M. Thyroid and corticosteroid signaling in amphibian metamorphosis. Cells 11:1595. doi.org/ 10.3390/cells11101595
2019
Sachs, L.M., Buchholz, D.R. Insufficiency of Thyroid Hormone in Frog Metamorphosis and the Role of Glucocorticoids. Front. Endocrinol. 10:287. doi: 10.3389/fendo.2019.00287
2018
Buchholz, D.R., Shi, Y.-B. Dual function model revised by thyroid hormone receptor alpha knockout frogs. Gen. Comp. Endocrinol. 265:214–218. doi.org/10.1016/j.ygcen.2018.04.020
2018
Buchholz, D.R., Shi, Y.-B. Methods for Investigating the Larval Period and Metamorphosis in Xenopus. Cold Spring Harb Protoc doi:10.1101/pdb.top097667
2017
Buchholz, D.R. Xenopus metamorphosis as a model to study thyroid hormone receptor function during vertebrate developmental transitions. Mol. Cell. Endocrinol. 459:64-70
2017
Sachs, L.M., Buchholz, D.R. Frogs Model Man: In-vivo thyroid hormone signaling during development. genesis55:e23000
2015
Buchholz, D.R. More similar than you think: Frog metamorphosis as a model of human perinatal endocrinology. Dev. Biol. 408:188–195
2014
Kulkarni, S.S., Buchholz, D.R. Corticosteroid signaling in frog metamorphosis. Gen. Comp. Endocrinol. 203:225–231
2013
Kulkarni, S.S., Buchholz, D.R. Developmental programs and endocrine disruption in frog metamorphosis: The perspective from microarray analysis. Curr. Top. Devel. Biol. 103:329-364
2012
Buchholz, D.R. Chapter 16: Tet-On binary systems for tissue-specific and inducible transgene expression, pp. 265-275. InXenopus Protocols: Post-Genomic Approaches, 2nd Ed. Eds Hoppler, S. and Vize, P. For series: Methods in Molecular Biology 917. New York: Humana Press
2011
Buchholz, D.R., Moakalik, C.L., Kulkarni, S.S., Hollar, A.R., Ng. A. Chapter 5: Hormonal regulation and the evolution of amphibian metamorphic diversity. In Mechanisms of Life History Evolution. pp. 87-97. Eds. Flatt, T. and Heyland, A. Oxford University Press
2006
Buchholz, D.R., Paul, B.D., Fu, L., Tomita, A., and Shi, Y.-B. Molecular and developmental analyses of thyroid hormone receptor function in Xenopus laevis, the African clawed frog. Gen. Comp. Endo. 145: 1-19
2006
Buchholz, D.R., Paul, B.D., and Shi, Y,-B. Chapter 11: Chromatin immunoprecipitation for in vivo studies of transcriptional regulation during development. In Analysis of Growth Factor Signaling in Embryos, pp. 257-271. Whitman, M. and Sater, A.K., eds. For "Methods in Signal Transduction" series, CRC Press
2003
Hsia, V. S.-C., Tomita, A., Obata, K., Paul, B., Buchholz, D.R., and Shi, Y.-B. Role of chromatin disruption and histone acetylation in thyroid hormone receptor action: Implications in the regulation of HIV-1 LTR. Histol. Histopathol. 18(1): 323-331
2001
Shi, Y.-B., Fu, L. Hsia, V., Tomita, A., Buchholz, D.R. Thyroid hormone regulation of apoptotic tissue remodeling during anuran metamorphosis. Cell Res. 11(4): 245-252